CRISPR-Cas9 alternatives

Prime editing is a 'search-and-replace' gene-editing technology that can make targeted insertions, deletions, and all 12 possible base-to-base changes without creating double-strand breaks, with AI models like OptiPrime and DeepPrime optimizing guide RNA design and predicting outcomes.

Base editing precisely converts one DNA base pair to another (e.g., C-G to T-A or A-T to G-C) without inducing double-strand DNA breaks, with AI used to engineer more compact and precise enzymes.

OpenCRISPR-1 is the first functional gene editor entirely designed by artificial intelligence using large language models, demonstrating comparable or improved activity and specificity over naturally occurring Cas9.

CRISPR-Cas12a is a smaller, single-guide RNA CRISPR nuclease that creates staggered-end DNA cuts, offering easier delivery and enhanced precision, with AI used to optimize gRNA design and predict outcomes.